PD-1 & PD-L1

EP-101-BLI

INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).